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Synthetic and Naive Human and Mouse Antibody Libraries
HuScL-1(R), HuScL-2(R), HuScL-3(R) and HuScL-4(R) Human Single Chain Antibody
Library are natural or synthetic libraries of a diversity of over 1.4×109.
We also have a HuFabL(R) Human Fab Antibody Library of a size of 2.0×109.
In addition, we have a MuScL(R) Murine Single Chain Antibody Library and a
MuFabL(R) Murine Fab Antibody Library.
o HuScL-1(R): Human Single Chain Antibody Library
1. Phagemid phage display system
2. Mono-display by pIX fusion
3. scFv format
4. Construction strategy: VH and VL genes were fully synthesized; mixture of 18 sub-libraries
5. Capacity: 1.35× 1010
6. Proliferation in TG1 cells
7. Helper phage: M13K07
o HuScL-2(R): Human Single Chain Antibody Library
1. Phagemid phage display system
2. M13 pIII fusion containing short His6 and c-Myc detection tag
3. VH-VL format scFv library
4. Mixed human antibody genes from naive donors and from semi-synthesis
5. Capacity: 1.42*109
6. Proliferation in TG1 cells
7. Production of soluble antibody fragments in
HB2151
8. Helper phage
KM13
o HuScL-3(R): Human Single Chain Antibody Library
1. Phagemid phage display system
2. M13 pIII fusion containing short His6 and V5 detection tags
3. VH-VL format scFv library
4.
Collections of human antibody genes from non-immunized donors;
5. VL/VH chain recombination through an integrated Cre/lox system
6. Theoretic capacity: 1.0*1016
7. Proliferation in TG1 cells
8. Production of soluble antibody fragments in HB2151
9. Helper phage M13KO7
o HuScL-4(R): Human Single Chain Antibody Library
1. Phagemid phage display system
2. M13 pIII fusion containing short c-Myc detection tag
3. VH-VL format scFv
library
4. Seven heavy chain frameworks and seven light chain frameworks representing the structural diversity of human antibody repertoire
5. Complete randomization in “HCDR3" and “LCDR3”
6. Capacity: 1.5*109
o HuFabL(R): Human Fab Antibody Library
1. Phagemid phage display system
2. M13 gIII fusion containing short His6 and c-Myc
detection tag
3. Fab format
4. Collections of human antibody genes from
non-immunized donors
5. 2.0*109
6. Proliferation in XL1-blue cells
7. Production of soluble antibody fragments in
TOP10F'
8. Helper phage VCSM13
o MuScL(R): Murine Single Chain Antibody Library
1.
Phagemid phage display system
2. M13 pIII fusion
3. scFv format
4. Collections of antibody
genes from na"ive mice
5. Capacity: 8.0×108
o MuFabL(R): Murine Fab Antibody Library
6. Phagemid phage display system
7. M13 gIII fusion
8. Fab format
9. Collections of antibody genes from na"ive mice
10. Capacity:
6.0*109
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